Partial Purification of the Protein Moiety of Porcine Tissue Thromboplastin
نویسندگان
چکیده
منابع مشابه
Partial purification of the protein moiety of porcine tissue thromboplastin.
The tissue thromboplastin activity of porcine lung was separated from an aqueous tissue extract by differential centrifugation. Repeated suspension and sedimentation yielded a thromboplastin preparation free of soluble protein. The most effective delipidation of the lyophilized preparation with minimum denaturation of the protein moiety of thromboplastin was achieved using n-heptane/n-butanol e...
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Purification and some properties of the protein component of tissue thromboplastin from human brain.
The protein component of tissue thromboplastib (Factor III) from human brain was purified by extraction of a microsomal fraction with sodium deoxycholate, gel filtration of the extract on Sephadex G-100 and preparative polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate. The product, apoprotein III, was homogeneous by anayltical polyacrylamide-gel electrophoresis, and ...
متن کاملImproved procedure for the purification of thromboplastin apoprotein from porcine brain
Thromboplastin (factor Ill) is a membrane-bound lipoprotein which initiates the extrinsic coagulation pathway when released from a latent state following tissue injury. This activation is brought about by the protein component (apoprotein 111) serving as a receptor for clotting factors VII and X. Apoprotein Ill, as well as the lipid component of thromboplastin, are both necessary for the expres...
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ژورنال
عنوان ژورنال: Pathophysiology of Haemostasis and Thrombosis
سال: 1977
ISSN: 1424-8832,1424-8840
DOI: 10.1159/000214192